PLA2G4A Knockout cell line (THP-1)

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PLA2G4A Knockout cell line (THP-1) Order

PLA2G4A Knockout cell line (THP-1)
Catalog#
YKO-HT0216
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PLA2G4A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PLA2G4A Knockout cell line (THP-1)
Species
Human
Gene Name
PLA2G4A
Gene ID
5321
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript PLA2G4A-201)
Validation: PCR+Sanger Sequencing
PLA2G4A Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PLA2G4A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PLA2G4A gene knockout PLA2G4A Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PLA2G4A Knockout cell line (THP-1)
PLA2G4A Knockout cell line (THP-1)
Catalog#
YKO-HT0216
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Catalog#: YKO-MT0038
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    Catalog#: YKO-H713
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    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
    YKO-HS3910(HeLa)

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    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Pla2g4a Knockout cell line (Raw 264.7)

    Pla2g4a Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Pla2g4a
    Gene ID: 18783
    Size: 1*10^6
    Catalog#: YKO-MT0038
      inquiry   More details
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
      inquiry   More details
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
      inquiry   More details

PLA2G4A Knockout cell line (THP-1) Order

PLA2G4A Knockout cell line (THP-1)
Catalog#
YKO-HT0216
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PLA2G4A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PLA2G4A Knockout cell line (THP-1)
Species
Human
Gene Name
PLA2G4A
Gene ID
5321
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript PLA2G4A-201)
Validation: PCR+Sanger Sequencing
PLA2G4A Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PLA2G4A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PLA2G4A gene knockout PLA2G4A Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PLA2G4A Knockout cell line (THP-1)
PLA2G4A Knockout cell line (THP-1)
Catalog#
YKO-HT0216
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • Pla2g4a Knockout cell line (Raw 264.7)

    Pla2g4a Knockout cell line (Raw 264.7)
      inquiry   More details

    Organism: Mouse
    Gene Name: Pla2g4a
    Gene ID: 18783
    Size: 1*10^6
    Catalog#: YKO-MT0038
    YKO-MT0038(Raw 264.7)
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)
      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
    YKO-H713(A549)
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
    YKO-HS3910(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • Pla2g4a Knockout cell line (Raw 264.7)

    Pla2g4a Knockout cell line (Raw 264.7)

    Organism: Mouse
    Gene Name: Pla2g4a
    Gene ID: 18783
    Size: 1*10^6
    Catalog#: YKO-MT0038
      inquiry   More details
  • PLA2G4A Knockout cell line (A549)

    PLA2G4A Knockout cell line (A549)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-H713
      inquiry   More details
  • PLA2G4A Knockout cell line (HeLa)

    PLA2G4A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: PLA2G4A
    Gene ID: 5321
    Size: 1*10^6
    Catalog#: YKO-HS3910
      inquiry   More details

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